Immortalization of canine adipose-derived mesenchymal stem cells and their seminiferous tubule transplantation.
Jia FangYudong WeiXin TengShanting ZhaoJinlian HuaPublished in: Journal of cellular biochemistry (2018)
Adipose-derived mesenchymal stem cells (ADSCs) are proven to provide good effects in numerous tissue engineering application and other cell-based therapies. However, the difficulty in the proliferation of ADSCs, known as the "Hayflick limit" in vitro, limits their clinical application. Here, we immortalized canine ADSCs (cADSCs) with SV40 gene and transplanted them into busulfan-induced seminiferous tubules of infertile mice. The proliferation of these immortalized cells was improved significantly. Then, cellular differentiation assays showed that the immortalized cADSCs could differentiate into three-germ-layer cells, osteogenesis, chondrogenesis, adipogenesis phenotypes, and primordial germ cell-like cells (PGCLCs). In addition, the immortalized cADSCs can proliferate in the busulfan-induced seminiferous tubules of infertile mice. These findings confirmed that the immortalized cADSCs maintain the criteria of cADSCs.
Keyphrases
- induced apoptosis
- germ cell
- tissue engineering
- high fat diet induced
- cell cycle arrest
- signaling pathway
- high glucose
- diabetic rats
- insulin resistance
- adipose tissue
- polycystic ovary syndrome
- endoplasmic reticulum stress
- drug induced
- allogeneic hematopoietic stem cell transplantation
- cell therapy
- stem cells
- single cell
- type diabetes
- gene expression
- endothelial cells
- transcription factor
- dna methylation
- mesenchymal stem cells
- skeletal muscle