Seaweed debris is susceptible to being buried in sediments due to natural environmental changes and human activities. So far, the effect of buried seaweeds on the environment and its decomposition mechanism remains unclear. This study simulated the decomposition of seaweed Gracilariopsis lemaneiformis for 180 days with different burial depths (0 cm and 10 cm) and burial weights (10 g and 20 g). Our findings revealed that compared with Gracilariopsis decomposition on the sediment surface, the seaweed buried in sediment slowed down the release of N, P, and dissolved organic carbon (DOC) by enhancing the activity of diverse anaerobic microbes (i.e. Draconibacterium, Desulfuromusa, Sediminispirochaeta), which were associated with organic matter decomposition. The enhanced burial quantity of Gracilariopsis resulted in a 3.28 % increase in sediment OC and enriched the humification degree of DOC in seawater. These results highlight the role of seaweed burial in enhancing OC sequestration in marine environments.