Extracellular vesicles and high-density lipoproteins: Exercise and oestrogen-responsive small RNA carriers.
Sira KarvinenTia-Marje KorhonenTero SievänenJari E KarppinenHanna-Kaarina JuppiVeera JakoahoUrho M KujalaJari A LaukkanenMaarit LehtiEija K LaakkonenPublished in: Journal of extracellular vesicles (2023)
Decreased systemic oestrogen levels (i.e., menopause) affect metabolic health. However, the detailed mechanisms underlying this process remain unclear. Both oestrogens and exercise have been shown to improve metabolic health, which may be partly mediated by circulating microRNA (c-miR) signalling. In recent years, extracellular vesicles (EV) have increased interest in the field of tissue crosstalk. However, in many studies on EV-carried miRs, the co-isolation of high-density lipoprotein (HDL) particles with EVs has not been considered, potentially affecting the results. Here, we demonstrate that EV and HDL particles have distinct small RNA (sRNA) content, including both host and nonhost sRNAs. Exercise caused an acute increase in relative miR abundancy in EVs, whereas in HDL particles, it caused an increase in transfer RNA-derived sRNA. Furthermore, we demonstrate that oestrogen-based hormonal therapy (HT) allows the acute exercise-induced miR-response to occur in both EV and HDL particles in postmenopausal women, while the response was absent in nonusers.
Keyphrases
- high density
- postmenopausal women
- cell proliferation
- long non coding rna
- high intensity
- liver failure
- long noncoding rna
- bone mineral density
- healthcare
- public health
- physical activity
- respiratory failure
- resistance training
- mental health
- aortic dissection
- health information
- nucleic acid
- stem cells
- type diabetes
- metabolic syndrome
- skeletal muscle
- cancer therapy
- risk assessment
- mesenchymal stem cells
- case control