Silk Fibroin Particles as Carriers in the Development of All-Natural Hemoglobin-Based Oxygen Carriers (HBOCs).
Marisa O PachecoHenry M LutzJostin ArmadaNickolas DaviesIsabelle K GerzenshteinAlaura S CakleyBruce D SpiessWhitney L StoppelPublished in: bioRxiv : the preprint server for biology (2023)
In this manuscript, we generate silk fibroin particles using an all-aqueous processing technique starting from silk fibroin polymer systems of differing molecular weights. We analyze the extent to which silk concentration and extraction time affect particle size. Further, we analyze the encapsulation of hemoglobin in the particle system and assess immune activation in macrophage-like cultures.