RNA-binding activity of TRIM25 is mediated by its PRY/SPRY domain and is required for ubiquitination.

Nila Roy ChoudhuryGregory HeikelMaryia TrubitsynaPeter KubikJakub Stanislaw NowakShaun WebbSander GrannemanChristos SpanosJuri RappsilberAlfredo CastelloGracjan Michlewski

Published in: BMC biology (2017)

Our results suggest that many other proteins with the PRY/SPRY domain could have yet uncharacterized RNA-binding potential. Together, our data reveal new insights into the molecular roles and characteristics of RNA-binding E3 ubiquitin ligases and demonstrate that RNA could be an essential factor in their enzymatic activity.

Keyphrases

nucleic acid
dna binding
binding protein
gene expression
genome wide
dna methylation
hydrogen peroxide
single cell
nitric oxide
machine learning
transcription factor
single molecule
data analysis