Solid Phase Peptide Synthesis on Chitosan Thin Films.
Tadeja KatanRupert KarglTamilselvan MohanTobias SteindorferMiran MozetičJanez KovačKarin Stana KleinschekPublished in: Biomacromolecules (2022)
Stable chitosan thin films can be promising substrates for creating nanometric peptide-bound polyglucosamine layers. Those are of scientific interest since they can have certain structural similarities to bacterial peptidoglycans. Such films were deposited by spin coating from chitosan solutions and modified by acetylation and N -protected amino acids. The masses of deposited materials and their stability in aqueous solutions at different pH values and water interaction were determined with a quartz crystal microbalance with dissipation (QCM-D). The evolution of the surface composition was followed by X-ray photoelectron (XPS) and attenuated total reflectance infrared (ATR-IR) spectroscopy. Morphological changes were measured by atomic force microscopy (AFM), while the surface wettability was monitored by by static water contact angle measurements. The combination of the characterization techniques enabled an insight into the surface chemistry for each treatment step and confirmed the acetylation and coupling of N -protected glycine peptides. The developed procedures are seen as first steps toward preparing thin layers of acetylated chitin, potentially imitating the nanometric peptide substituted glycan layers found in bacterial cell walls.
Keyphrases
- atomic force microscopy
- drug delivery
- single molecule
- high resolution
- room temperature
- high speed
- amino acid
- wound healing
- hyaluronic acid
- solar cells
- cell therapy
- single cell
- computed tomography
- molecular docking
- density functional theory
- histone deacetylase
- magnetic resonance imaging
- oxidative stress
- combination therapy
- molecular dynamics
- ionic liquid
- bone marrow
- cell surface
- fine needle aspiration
- carbon nanotubes
- transition metal