Prediction models for evaluating the impacts of ambient air pollutants on the biochemical response of selected tree species of Haridwar, India.

This study aimed to assess the spatio-temporal impact of selected ambient air pollutants (SO 2 , NO 2 , PM 10 , and PM 2.5 ) on the biochemical response of four tree species including Neem (Azadirachta indica), Mountain cedar (Toona ciliate), Bottlebrush (Callistemon citrinus), and Guava (Psidium guajava) in the province of Haridwar City, Uttarakhand, India. The study was performed in 2020 and 2021 over three selected sites (S1: institutional; S2: industrial; and S3: urban). Purposely, seasonal data of ambient air pollutants and biochemical parameters (ascorbic acid, carotenoid, chlorophyll, pH, relative water content, and dust load) of selected tree species were collected and analyzed using multiple linear regression (MLR) tool to develop prediction models. The results indicated that biochemical parameters of all tree species were negatively impacted by the polluted ambient air quality in the industrial and urban (S2 and S3) sites as compared to the non-polluted institutional (S1) site. The models were characterized by high prediction performance as indicated by the coefficient of determination (R 2 ) values greater than 0.80. Moreover, A. indica was found to be more 'tolerant' based on the air pollution tolerance index (APTI) followed by T. ciliate, P. guajava, and C. citrinus. Similarly, the anticipated performance index (API) was reported higher for A. indica (75 to 81.25%) followed by T. ciliate (68.75 to 75.00%), P. guajava (56.25%), and C. citrinus (37.50%), respectively. This study revealed that the selected tree species are being negatively impacted by the induced pollutant exposure in the urban and industrial region of Haridwar, India which needs sufficient mitigation measures to conserve their diversities.