One-pot fluorescent assay for sensitive detection of APOBEC3A activity.

Bingyao WangXiong ZhangYafen WangKun ChenFang WangXiaocheng Weng Xiang Zhou

Published in: RSC chemical biology (2021)

We reported a one-pot fluorescence-based assay to quantitively detect A3A activity combined with cytosine deamination and uracil excision. After deamination by A3A and USER enzyme treatment, the fluorescent turn-on effect at 520 nm was observed, which can be used to evaluate the A3A activity and screen inhibitors.

Keyphrases

sensitive detection
quantum dots
high throughput
living cells
photodynamic therapy
fluorescent probe
energy transfer
single cell
loop mediated isothermal amplification
combination therapy