The Zap1 transcriptional activator negatively regulates translation of the RTC4 mRNA through the use of alternative 5' transcript leaders.
Amanda J BirdSimon LabbéPublished in: Molecular microbiology (2017)
The zinc-responsive transcription activator Zap1 plays a central role in zinc homeostasis in the budding yeast Saccharomyces cerevisiae. In zinc-deficient cells, Zap1 binds to zinc responsive elements in target gene promoters and activates gene expression. In most cases, Zap1-dependent gene activation results in increased levels of mRNAs and proteins. However, Zap1-dependent activation of RTC4 results in increased levels of the RTC4 mRNA and decreased levels of the Rtc4 protein. This atypical regulation results from Zap1-mediated changes in the transcriptional start site for RTC4 and the production of a RTC4 transcript with a longer 5' leader. This long RTC4 transcript contains small upstream open reading frames that prevent translation of the downstream RTC4 ORF. The new studies with Zap1 highlight how a transcriptional activator can facilitate decreased protein expression.
Keyphrases
- gene expression
- saccharomyces cerevisiae
- transcription factor
- oxide nanoparticles
- nuclear factor
- rna seq
- dna methylation
- genome wide
- copy number
- induced apoptosis
- cancer therapy
- binding protein
- minimally invasive
- genome wide identification
- small molecule
- oxidative stress
- immune response
- drug delivery
- single cell
- cell death
- signaling pathway
- toll like receptor
- protein protein