Freezing Diluted Bovine Serum Albumin Standards Does Not Significantly Affect Standard Curves.

Freezing diluted BSA standards at either -20°C or -80°C and thawing and refreezing them up to three times does not significantly alter their protein concentrations.Inter-person variability in standard curves is greater than intra-person variability, and controlling for developer decreases variability in most cases.There is more consistency in standard curves when a single batch of diluted standards is aliquoted and frozen at either -20°C or -80°C and thawed up to three times than when the same investigator or different investigators make fresh standards before each assay.