Collagen Sandwich Culture of Primary Hepatocytes for Image-Based Investigations.
Dong FuPublished in: Methods in molecular biology (Clifton, N.J.) (2022)
Collagen sandwich culture of hepatocytes retains many in vivo-like properties and is used for many investigations in liver cell biology, and hepatic pharmacology and toxicology. This chapter describes the method of establishing collagen sandwich culture of hepatocytes in a glass bottom dish for image-based studies.