Two-Photon Fluorescence Probe for Selective Monitoring of Superoxide in Live Cells and Tissues.
Liyan ChenMyoung Ki ChoDi WuHwan Myung KimJuyoung YoonPublished in: Analytical chemistry (2019)
The abnormal location or generation of superoxide radical anion (O2•-) are implicated in many diseases, including cancers; thus, development of an efficient method to detect O2•- is of great importance. Inspired by the fluorophore-governed selective manner to O2•- and peroxynitrite (ONOO-) of previously reported phosphinate-based fluorescence probes, in this contribution, a phosphinothioate-containing probe, TPP, was designed. The probe exhibited easy accessibility through a one-step sequence and good photostability and biocompatibility. Interestingly, TPP showed high specificity and sensitivity to O2•- over other reactive oxygen species/nitrogen species including ONOO-. Furthermore, with the assistance of two-photon microscopy, TPP was successfully applied for imaging endogenous O2•- in live cells and tissues.
Keyphrases
- living cells
- single molecule
- fluorescent probe
- induced apoptosis
- cell cycle arrest
- reactive oxygen species
- gene expression
- high resolution
- hydrogen peroxide
- quantum dots
- cell death
- small molecule
- endoplasmic reticulum stress
- signaling pathway
- nitric oxide
- pi k akt
- optical coherence tomography
- cell proliferation
- fluorescence imaging
- ionic liquid
- amino acid
- young adults