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Demonstration of microRNA using in situ hybridisation on formalin fixed paraffin wax samples using conventional oligonucleotide probes: a comparison with the use of locked nucleic acid probes.

A WarfordN S RahmanD A RibeiroPinar Uysal-Onganer
Published in: British journal of biomedical science (2020)
This study has shown that simplification of ISH protocols by the use of COPs provides equivalent results to the use of LNA methods and it can be used to precisely identify cells in which miRNAs are expressed.
Keyphrases
  • nucleic acid
  • induced apoptosis
  • living cells
  • single molecule
  • fluorescence imaging
  • cell cycle arrest
  • fluorescent probe