Measuring autophagosome flux.
Andre du ToitJan-Hendrik S HofmeyrThomas J GniadekBen LoosPublished in: Autophagy (2018)
ATG5/APG5, autophagy-related 5; GFP, green fluorescent protein; LAMP1, lysosomal-associated membrane protein 1; MAP1LC3/LC3, microtubule-associated protein 1 light chain 3; J, flux; MEF, mouse embryonic fibroblast; MTOR, mechanistic target of rapamycin kinase; nA, number of autophagosomes; nAL, number of autolysosomes; nL, number of lysosomes; p-MTOR, phosphorylated mechanistic target of rapamycin kinase; RFP, red fluorescent protein; siRNA, small interfering RNA; τ, transition time; TEM, transmission electron microscopy.
Keyphrases
- electron microscopy
- quantum dots
- cell proliferation
- living cells
- simultaneous determination
- protein protein
- protein kinase
- cell death
- tyrosine kinase
- amino acid
- mass spectrometry
- oxidative stress
- signaling pathway
- binding protein
- label free
- cancer therapy
- small molecule
- fluorescent probe
- drug delivery
- solid phase extraction
- loop mediated isothermal amplification
- high resolution mass spectrometry
- wound healing