Application of WST-8 based colorimetric NAD(P)H detection for quantitative dehydrogenase assays.

Kamonwan ChamchoyDanaya PakotipraphaPornpan PumiratUbolsree LeartsakulpanichUsa Boonyuen

Published in: BMC biochemistry (2019)

The results suggest that the WST-8 assay is a sensitive and rapid method for determining NAD(P)H concentration and dehydrogenase enzyme activity, which can be further applied for the high-throughput screening of dehydrogenases.

Keyphrases

loop mediated isothermal amplification
high throughput
sensitive detection
gold nanoparticles
label free
high resolution
fluorescent probe
real time pcr
single molecule