The synthesis and characterization of glutathione-modified superparamagnetic iron oxide nanoparticles and their distribution in rat brains after injection in substantia nigra.
Guihua HanBaolin ZhangHao ZhangDong HanJie TanBoning YangPublished in: Journal of materials science. Materials in medicine (2018)
Glutathione-modified superparamagnetic iron oxide nanoparticles (GSH-SPIONs) were prepared by conjugating glutathione (GSH) on the surface of the PEG (Polyethylene glycol)/PEI (polyethyleneimine)-SPIONs which were synthesized by thermal decomposition method. Thermogravimetric analysis showed that the mass fraction of GSH on the surface of SPIONs was 30.64 wt%. GSH-SPIONs in PBS were injected into the substantia nigra of rat brains. The subcellular distributions of the nanoparticles in the brains was examined by the transmission electron microscope (TEM). A remarkable amount of GSH-SPIONs were found in vesicles inside cell bodies and axons, and in mitochondria. TEM pictures show that GSH-SPIONs enter the neuronal cells by endocytosis and travel through axoplasmic transport. GSH-SPIONs have great potential as drug delivery agents in the brain to treat diseases or study brain function via mitochondria-targeting way or axoplasmic transport way.
Keyphrases
- iron oxide nanoparticles
- fluorescent probe
- drug delivery
- induced apoptosis
- oxidative stress
- cell death
- cancer therapy
- white matter
- single cell
- cerebral ischemia
- resting state
- climate change
- reactive oxygen species
- multiple sclerosis
- blood brain barrier
- risk assessment
- ultrasound guided
- cell proliferation
- endoplasmic reticulum
- endoplasmic reticulum stress