Quantification of Au Nanoparticle Biouptake and Distribution to Freshwater Algae Using Single Cell - ICP-MS.
Ruth C MerrifieldC StephanJ R LeadPublished in: Environmental science & technology (2018)
Quantifying metal and nanoparticle (NP) biouptake and distribution on an individual cellular basis has previously been impossible, given available techniques which provide qualitative data that are laborious to acquire and prone to artifacts. Quantifying metal and metal NP uptake and loss processes in environmental organisms will lead to mechanistic understanding of biouptake and improved understanding of potential hazards and risks of metals and NPs. In this work, we present a new technique, single cell inductively coupled plasma mass spectrometry (SC-ICP-MS), which allows quantification of metal concentrations on an individual cell basis down to the attogram (ag) per cell level. We present data validating the novel method, along with the mass of metal per cell. Finally, we use SC-ICP-MS, with ancillary cell counting methods, to quantify the biouptake and strong sorption and distribution of both dissolved Au and Au NPs in a freshwater alga (Cyptomonas ovate). The data suggests differences between dissolved and NP uptake and loss. In the case of NPs, there was a dose and time dependent uptake, but individual cellular variations; at the highest realistic exposure conditions used in this study up to 40-50% of cells contained NPs, while 50-60% of cells did not.
Keyphrases
- single cell
- mass spectrometry
- rna seq
- multiple sclerosis
- cell therapy
- induced apoptosis
- ms ms
- electronic health record
- human health
- high throughput
- big data
- stem cells
- high resolution
- oxidative stress
- liquid chromatography
- endoplasmic reticulum stress
- systematic review
- organic matter
- cell death
- machine learning
- quantum dots
- reduced graphene oxide
- capillary electrophoresis
- gold nanoparticles
- multidrug resistant
- artificial intelligence
- gas chromatography
- image quality
- health risk assessment
- heavy metals