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A Fast and Easy Method for Specific Detection of Circular RNA by Rolling-Circle Amplification.

Marcel BossChristoph Arenz
Published in: Chembiochem : a European journal of chemical biology (2019)
Circular RNAs (circRNAs) represent a new class of usually noncoding transcripts with largely unknown functions. Their research is hampered not least by the inapplicability of traditional analytical methods. Herein we describe a rapid and easy assay for the detection of natural circRNA, based on rolling-circle amplification (RCA). This technique does not require the use of fluorescently labeled RNA or DNA and can specifically detect circular RNA in the presence of a 1000-fold excess of the same linear RNA. Only standard devices such as (quantitative) PCR cyclers and gel electrophoresis are used.
Keyphrases
  • nucleic acid
  • loop mediated isothermal amplification
  • label free
  • real time pcr
  • high throughput
  • high resolution
  • circulating tumor
  • single molecule
  • pet ct