Quantifying protein dynamics and stability in a living organism.
Ruopei FengMartin GruebeleCaitlin M DavisPublished in: Nature communications (2019)
As an integral part of modern cell biology, fluorescence microscopy enables quantification of the stability and dynamics of fluorescence-labeled biomolecules inside cultured cells. However, obtaining time-resolved data from individual cells within a live vertebrate organism remains challenging. Here we demonstrate a customized pipeline that integrates meganuclease-mediated mosaic transformation with fluorescence-detected temperature-jump microscopy to probe dynamics and stability of endogenously expressed proteins in different tissues of living multicellular organisms.
Keyphrases
- single molecule
- induced apoptosis
- cell cycle arrest
- high resolution
- high throughput
- energy transfer
- gene expression
- endothelial cells
- endoplasmic reticulum stress
- signaling pathway
- computed tomography
- big data
- mass spectrometry
- oxidative stress
- machine learning
- cell therapy
- bone marrow
- label free
- multidrug resistant
- small molecule
- amino acid
- pet ct