Oxidative Stress and DNA methylation in male rat pups provoked by the transplacental and translactational exposure to bisphenol A.
Hanan M A El HenafyMarwa A IbrahimSamy A Abd El AzizEman M GoudaPublished in: Environmental science and pollution research international (2019)
The epigenetic changes induced by environmental contaminants play important roles in the inheritance of male reproductive dysfunction. The present study investigated DNA methylation changes and some oxidative stress biomarkers induced by bisphenol A (BPA) in male offspring. A total number of 48 female albino rats were administered orally with 50 μg/kg of BPA/day during gestation and/or lactation periods. At postnatal day 60, the samples were collected from the male pups to assess the serum testosterone, malondialdehyde (MDA) level, superoxide dismutase (SOD), glutathione S-transferase, and glutathione peroxidase (GSH-Px) activities in testicular tissue. DNA methylation in both DNA (cytosine-5)-methyltransferase 3A and estrogen receptor alpha genes was detected by methylation-specific PCR. BPA exposure resulted in significant decrease in the anogenital distance, testis and epididymis weights, serum testosterone level, SOD, GST, and GSH-Px levels with significant increase in weaning body weight and the MDA level. Additionally, BPA caused marked hypermethylation within Dnmt3A and ER- ∝ genes promoter regions in the testis of rat male pups. Graphical abstract.
Keyphrases
- dna methylation
- genome wide
- oxidative stress
- estrogen receptor
- gene expression
- body weight
- preterm infants
- dna damage
- breast cancer cells
- copy number
- diabetic rats
- hydrogen peroxide
- replacement therapy
- germ cell
- induced apoptosis
- cell cycle arrest
- mechanical ventilation
- high resolution
- extracorporeal membrane oxygenation
- signaling pathway
- cell free
- genome wide identification
- bioinformatics analysis
- human health
- heat stress
- circulating tumor cells