Self-assembly of DNA-gold nanoaggregate for visual detection of thymidine kinase 1 (TK1) mRNA via lateral flow assay.
Jing WangLu ShiQiaorong TangXinyu ZhuMengmeng WuWei LiuBaoxin LiYan JinPublished in: Mikrochimica acta (2023)
Nucleic acid lateral flow assay (NALFA) with gold nanoparticles (AuNPs) as colorimetric probes have been extensively adopted for point-of-care testing (POCT). However, the sensitivity of NALFA still needs to be improved. Herein, DNA-gold nanoaggregate (DNA-AuNA) was assembled as a signal amplification probe of NALFA for sensitive detection of tumor marker TK1 mRNA. Four functional oligonucleotides with complementary pairs were assembled to form DNA-AuNA that coupled more AuNPs to improve sensitivity. Thus, the limit of detection (LOD) was 0.36 pM, which is lower than that of conventional AuNPs-based NALFA. Moreover, the bioassay showed good reproducibility, stability, and specificity for detecting TK1 mRNA. The detection of TK1 mRNA in human serum was also satisfactory. Therefore, DNA-AuNA-based NALFA provides a sensitive method for portable detection of TK1 mRNA.
Keyphrases
- nucleic acid
- loop mediated isothermal amplification
- sensitive detection
- circulating tumor
- gold nanoparticles
- single molecule
- cell free
- label free
- real time pcr
- binding protein
- high throughput
- living cells
- small molecule
- multidrug resistant
- particulate matter
- fluorescence imaging
- polycyclic aromatic hydrocarbons
- aqueous solution