Single-cell transcriptional landscape of temporal neutrophil response to burn wound in larval zebrafish.
Yiran HouParth KhatriJulie RindyZachery SchultzAnqi GaoZhili ChenAngela L F GibsonAnna HuttenlocherHuy Q DinhPublished in: bioRxiv : the preprint server for biology (2024)
Neutrophils accumulate early in tissue injury. However, the cellular and functional heterogeneity of neutrophils during homeostasis and in response to tissue damage remains unclear. Here, we use larval zebrafish to understand neutrophil responses to thermal injury. Single-cell transcriptional mapping of myeloid cells during a 3-day time course in burn and control larvae revealed distinct neutrophil subsets and their cell-cell interactions with macrophages across time and conditions. The trajectory formed by three zebrafish neutrophil subsets resembles human neutrophil maturation, with varying transition patterns between conditions. Through ligand-receptor cell-cell interaction analysis, we found neutrophils communicate more in burns in a pathway and temporal manner. Finally, we identified the correlation between zebrafish myeloid signatures and human burn severity, establishing GPR84+ neutrophils as a potential marker of early innate immune response in burns. This work builds the molecular foundation and a comparative single-cell genomic framework to identify neutrophil markers of tissue damage using model organisms.
Keyphrases
- single cell
- rna seq
- immune response
- high throughput
- endothelial cells
- cell therapy
- dendritic cells
- acute myeloid leukemia
- oxidative stress
- stem cells
- transcription factor
- high resolution
- mesenchymal stem cells
- signaling pathway
- induced apoptosis
- cell proliferation
- risk assessment
- mass spectrometry
- binding protein
- inflammatory response