Metagenomics of Wastewater Influent from Wastewater Treatment Facilities across Ontario in the Era of Emerging SARS-CoV-2 Variants of Concern.

Opeyemi Uwangbaoje LawalLinkang ZhangValeria R ParreiraR Stephen BrownCharles ChettleburghNora DannahRobert DelatollaKimberly A GilbrideTyson E GraberGolam IslamJames KnocklebySean MaHanlan McDougallRobert Michael L McKayAleksandra MloszewskaClaire OswaldMark ServosMegan Swinwood-SkyGustavo YbazetaMarc HabashLawrence Goodridge

Published in: Microbiology resource announcements (2022)

We report metagenomic sequencing analyses of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) RNA in composite wastewater influent from 10 regions in Ontario, Canada, during the transition between Delta and Omicron variants of concern. The Delta and Omicron BA.1/BA.1.1 and BA.2-defining mutations occurring in various frequencies were reported in the consensus and subconsensus sequences of the composite samples.

Keyphrases

wastewater treatment
sars cov
respiratory syndrome coronavirus
antibiotic resistance genes
copy number
coronavirus disease
single cell
clinical practice
genome wide
genetic diversity