Flow cytometric basophil activation tests: Staining of exteriorized basophil granule matrix by fluorescent avidin versus appearance of CD63.

Didier Gaston Ebo Jessy Elst Michel Van Houdt Isabel PintelonJean-Pierre TimmermansTatsuo Horiuchi Margaretha Antje Faber Margo Maria Hagendorens Christel H Mertens Vito Sabato

Published in: Cytometry. Part B, Clinical cytometry (2020)

Staining of exteriorized proteoglycans by avidin is a reliable technique to quantify basophil degranulation but offers no added value when compared to traditional assays that use CD63 as a readout.

Keyphrases

nk cells
flow cytometry
high throughput
quantum dots
label free