Concordance in detection of microsatellite instability by PCR and NGS in routinely processed tumor specimens of several cancer types.
Stephan BartelsIsabel GroteMadeleine WagnerJannik BoogElisa SchipperTanja Reineke-PlaassHans KreipeUlrich LehmannPublished in: Cancer medicine (2023)
MSI analysis of FFPE DNA by NGS is feasible and the results show a high concordance in comparison with the monomorphic marker MSI-PCR. However, cases with a subtle MSI+ phenotype, most frequently manifest in EC, have a risk of a false-negative result by NGS and should be preferentially analyzed by capillary electrophoresis.