Unambiguous Identification of Glucose-Induced Glycation in mAbs and other Proteins by NMR Spectroscopy.
Jennifer E MoisesChristof ReglArthur HinterholzerChristian G HuberMario SchubertPublished in: Pharmaceutical research (2022)
Based on this unique correlation pattern, 2D NMR spectroscopy can be used to unambiguously identify glucose-induced glycation in any protein of interest. We provide a robust method that is orthogonal to MS-based methods and can also be used for cross-validation.