Adopting Duplex Sequencing™ Technology for Genetic Toxicity Testing: A Proof-of-Concept Mutagenesis Experiment with N-Ethyl-N-Nitrosourea (ENU)-Exposed Rats.

Stephanie L Smith-RoeCheryl A HobbsVictoria HullJ Todd AumanLeslie RecioMichael A StreickerMiriam V RivasGabriel A PrattFang Yin LoJacob E HigginsElizabeth K SchmidtLindsey N WilliamsDaniela NachmansonCharles C ValentineJesse J SalkKristine L Witt

Published in: bioRxiv : the preprint server for biology (2023)

DuplexSeq is an ultra-accurate NGS technology that directly quantifies mutationsENU-dependent mutagenesis was detected 24 h post-exposure in proliferative tissuesMultiple tissues exhibited the canonical ENU mutation spectrum 7 d after exposureResults obtained with DuplexSeq were highly concordant between laboratoriesThe Rat-50 Mutagenesis Assay is promising for applications in genetic toxicology.

Keyphrases

crispr cas
genome wide
oxidative stress
high resolution
gene expression
copy number
high throughput
single cell
ionic liquid