Autofluorescence prediction model for fluorescence unmixing and age determination.
Marco EigenfeldRoland KerpesIain WhiteheadThomas BeckerPublished in: Biotechnology journal (2022)
The proposed approach of single-laser spectral unmixing and subsequent Gaussian mixture analysis showed that the microscopy data was consistent with the unmixed fluorescence spectra. The demonstrated approach enables fast and reliable unmixing of flow cytometric spectral data using a single-laser spectral unmixing method. This analysis method enables age determination of cells in industrial processes. This age determination allows for quantifying the yeast cell's age fractions, providing a detailed view of age-related changes. Additionally, the bud scar labeling technique can be used to determine age-related changes in Pichia pastoris yeast for biotechnological applications or recombinant protein expression.
Keyphrases
- optical coherence tomography
- single molecule
- solid phase extraction
- electronic health record
- induced apoptosis
- molecularly imprinted
- high resolution
- single cell
- heavy metals
- saccharomyces cerevisiae
- magnetic resonance imaging
- cell therapy
- signaling pathway
- density functional theory
- artificial intelligence
- data analysis
- mass spectrometry
- high throughput
- endoplasmic reticulum stress
- wound healing
- simultaneous determination
- cell wall
- molecular dynamics