Utilizing minimally purified secreted rAAV for rapid and cost-effective manipulation of gene expression in the CNS.

Marshall S GoodwinCara L CroftHunter S FutchDaniel RyuCarolina Ceballos-DiazXuefei LiuGiavanna PaternoCatalina MejiaDoris DengKimberly MenezesLaura LondonoKefren ArjonaMary ParianosVan TruongEva RostonicsAmanda HernandezSanford L BoyeShannon E BoyeYona LevitesPedro E CruzGriffin Golde

Published in: Molecular neurodegeneration (2020)

Unpurified rAAV vectors secreted into the media can efficiently transduce brain cells in vitro and in vivo, providing a cost-effective way to manipulate gene expression. The use of unpurified virus will greatly reduce costs of exploratory studies and further increase the utility of rAAV vectors for standard laboratory use.

Keyphrases

gene expression
dna methylation
induced apoptosis
cell cycle arrest
gene therapy
oxidative stress
cell death
case control
loop mediated isothermal amplification
sensitive detection