Structural Characterization of Polygonatum Cyrtonema Polysaccharide and Its Immunomodulatory Effects on Macrophages.
Ruiding WenLu LuoRuncheng ZhangXu-Dong ZhouWei WangLimin GongPublished in: Molecules (Basel, Switzerland) (2024)
A neutral Polygonatum cyrtonema polysaccharide (NPCP) was isolated and purified from Polygonatum cyrtonema by various chromatographic techniques, including DEAE-52 and Sephadex-G100 chromatography. The structure of NPCP was characterized by HPLC, HPGPC, GC-MS, FT-IR, NMR, and SEM. Results showed that NPCP is composed of glucose (55.4%) and galactose (44.6%) with a molecular weight of 3.2 kDa, and the sugar chain of NPCP was →1)-α-D-Glc-(4→1)-β-D-Gal-(3→. In vitro bioactivity experiments demonstrated that NPCP significantly enhanced macrophages proliferation and phagocytosis while inhibiting the M1 polarization induced by LPS as well as the M2 polarization induced by IL-4 and IL-13 in macrophages. Additionally, NPCP suppressed the secretion of IL-6 and TNF-α in both M1 and M2 cells but promoted the secretion of IL-10. These results suggest that NPCP could serve as an immunomodulatory agent with potential applications in anti-inflammatory therapy.
Keyphrases
- anti inflammatory
- mass spectrometry
- induced apoptosis
- simultaneous determination
- rheumatoid arthritis
- ms ms
- magnetic resonance
- tandem mass spectrometry
- high resolution
- inflammatory response
- cell cycle arrest
- metabolic syndrome
- bone marrow
- mesenchymal stem cells
- risk assessment
- endoplasmic reticulum stress
- water soluble