Enzymatic glycoengineering-based spin labelling of cell surface sialoglycans to enable their analysis by electron paramagnetic resonance (EPR) spectroscopy.

Mohit JaiswalTrang T TranJiatong GuoMingwei ZhouJosefina Garcia DiazGail E Fanucci Zhongwu Guo

Published in: The Analyst (2022)

A novel method for spin labelling of sialoglycans on the cell surface is described. C9-Azido sialic acid was linked to glycans on live cells via CSTII-catalysed α2,3-sialylation utilizing azido-sialic acid nucleotide as a sialyl donor, which was followed by attachment of a spin label to the azide via click reaction. It enables the study of cell surface sialoglycans by EPR spectroscopy.

Keyphrases

cell surface
single molecule
room temperature
density functional theory
induced apoptosis
high resolution
transition metal
cell cycle arrest
solid state
hydrogen peroxide
endoplasmic reticulum stress