Phycobiliproteins Ameliorate Gonadal Toxicity in Male Mice Treated with Cyclophosphamide.
Jorge Briseño-BugarínIsabel Hernández-OchoaXelha Araujo-PadillaMaría Angélica Mojica-VillegasRicardo Iván Montaño-GonzálezGabriela Gutiérrez-SalmeánChamorro-Cevallos GermánPublished in: Nutrients (2021)
Cyclophosphamide (CP)-which is used to treat autoimmune diseases and cancer-is related to gonadotoxicity attributed to oxidative stress. As phycobiliproteins (PBPs) are strong antioxidants that are unexplored as protective agents against male gonadotoxicity, our work aimed to investigate the effects of PBP crude extract on testicular damage and sperm parameter alterations caused by CP in mice. Three doses of PBP (50, 100, and 200 mg/kg) were tested in the experimental groups (n = 8 per group), administered concomitantly with 100 mg/kg CP. After 42 days receiving PBP daily and CP weekly, body and relative testicular weights, serum testosterone levels, testicular lipoperoxidation and antioxidant enzyme activity levels, and testicular histology and sperm parameter alterations were assessed. The results showed that PBP crude extract at 200 mg/kg prevented testosterone serum reduction, body weight loss, lipoperoxidation and enzyme activity increments, and sperm parameter alterations and partially ameliorated relative testicular weight reductions and histological damage in CP-treated mice. In conclusion, we showed that PBP crude extract (200 mg/kg) mitigated oxidative damage in the testes and ameliorated alterations in sperm parameters in mice treated with CP (100 mg/kg); therefore, PBP extract could be considered as a potential protective agent against CP toxicity.
Keyphrases
- oxidative stress
- germ cell
- weight loss
- diabetic rats
- dna damage
- ischemia reperfusion injury
- induced apoptosis
- high fat diet induced
- low dose
- high dose
- body mass index
- bariatric surgery
- insulin resistance
- young adults
- squamous cell carcinoma
- replacement therapy
- roux en y gastric bypass
- risk assessment
- papillary thyroid
- signaling pathway
- endoplasmic reticulum stress
- heat shock
- human health
- wild type
- lymph node metastasis