Using chitosan-coated magnetite nanoparticles as a drug carrier for opioid delivery against breast cancer.
Shima AliebrahimiAmir Farnoudian-HabibiFatemeh HeidariAmir AmaniVahideh MontazeriShiva Sabz AndamReza SaberAli Mohammad AlizadehSeyed Nasser OstadPublished in: Pharmaceutical development and technology (2024)
Over the past decades, opium derivatives have been discovered as new anticancer agents. In our study, Fe 3 O 4 superparamagnetic nanoparticles (SPIONs) decorated with chitosan were loaded with papaverine or noscapine to surmount drug delivery-related obstacles. Modifying the magnetic nanoparticles (MNP) surface with polymeric materials such as chitosan prevents oxidation and provides a site for drug linkage, which renders them a great drug carrier. The obtained systems were characterized by DLS (20-40 nm were achieved for MNPs and drug- loaded MNPs), TEM (spherical with average size of 11-20 nm) FTIR, XRD, and VSM (71.3 - 42.8 emu/g). Contrary to noscapine, papaverine-MNPs attenuated 4T1 murine breast cancer cell proliferation (11.50 ± 1.74 µg/mL) effectively compared to the free drug (62.35 ± 2.88 µg/mL) while sparing L-929 fibroblast cells (138.14 ± 4.38 µg/mL). Furthermore, SPION and SPION-chitosan displayed no cytotoxic activity. Colony-formation assay confirmed the long-term cytotoxicity of nanostructures. Both developed formulations promoted ROS production accompanied by late apoptotic cell death. The biocompatible nanoparticle exerted an augmenting effect to deliver papaverine to metastatic breast cancer cells.
Keyphrases
- drug delivery
- cell death
- cancer therapy
- wound healing
- drug release
- cell proliferation
- squamous cell carcinoma
- adverse drug
- photodynamic therapy
- magnetic nanoparticles
- breast cancer cells
- small cell lung cancer
- drug induced
- dna methylation
- signaling pathway
- high throughput
- hydrogen peroxide
- ionic liquid
- pain management
- reduced graphene oxide
- minimally invasive
- single cell
- endoplasmic reticulum stress
- quantum dots
- pi k akt