Exploiting orthology and de novo transcriptome assembly to refine target sequence information.

Julia F SöllnerGermán LeparcMatthias ZwickTanja SchönbergerTobias HildebrandtKay NieseltEric Simon

Published in: BMC medical genomics (2019)

In summary, we show how to refine protein sequences using RNA-Seq data and sequence information from closely related species. With the a&o-tool we provide a fully automated pipeline to perform refinement including cDNA translation and multiple sequence alignment for visual inspection. The major prerequisite for applying the a&o-tool is high quality sequencing data.

Keyphrases

rna seq
single cell
high throughput
amino acid
electronic health record
big data
machine learning
health information
deep learning
gene expression
genetic diversity
healthcare
binding protein
artificial intelligence