Phytol as an anticarcinogenic and antitumoral agent: An in vivo study in swiss mice with DMBA-Induced breast cancer.
Marcus Vinícius Oliveira Barros de AlencarMuhammad Torequl IslamRosália Maria Torres de LimaMárcia Fernanda Correia Jardim PazAntonielly Campinho Dos ReisAna Maria Oliveira Ferreira da MataJosé Williams Gomes de Oliveira FilhoGilberto Santos CerqueiraPaulo Michel Pinheiro FerreiraJoão Marcelo de Castro E SousaMohammad S MubarakAna Amélia de Carvalho Melo-CavalcantePublished in: IUBMB life (2018)
Phytol (PHY) (3,7,11,15-tetramethylhexadec-2-en-1-ol) exhibits various pharmacological properties including toxicity and cytotoxicity, and exerts antitumor activity. Owing to the urgent need of new pharmaceutical formulations for breast cancer therapy, this study aimed at the evaluation of antitumor activity of PHY in 7,12-dimethylbenzanthracene-cancer-induced animal model. Comet assay was employed to evaluate the cytogenetics, DNA repair, and antigenotoxic activities of PHY in neoplastic (breast) and non-neoplastic rodent cells (bone marrow, lymphocytes, and liver). Additionally, hematological, biochemical, histopathological, and immunohistochemical analyses were carried out in experimental animals. Thirty nonpregnant female mice (n = 5) underwent 7 weeks treatment with 6 mg/kg pro-carcinogen, PHY (4 mg/kg), and cyclophosphamide (25 mg/kg). Induction of cancer was confirmed by histopathology and immunohistochemistry for Ki-67. Results suggest that PHY exhibits low toxicity in comparison with other groups in hematological, biochemical, histopathological, and organ size parameters. Additionally, PHY showed modulatory effects on the pro-carcinogen, and induced genotoxicity and apoptosis in breast cancer cells. Furthermore, it showed a DNA damage repair capacity in mouse lymphocytes. These data indicate that PHY may have the potential as an anticancer candidate in pharmaceutical consumption. © 2018 IUBMB Life, 71(1):200-212, 2019.
Keyphrases
- dna damage
- dna repair
- oxidative stress
- diabetic rats
- high glucose
- bone marrow
- cancer therapy
- cell cycle arrest
- induced apoptosis
- papillary thyroid
- cell death
- type diabetes
- drug induced
- endoplasmic reticulum stress
- low dose
- dna damage response
- lymph node
- adipose tissue
- electronic health record
- neoadjuvant chemotherapy
- high dose
- radiation therapy
- skeletal muscle
- artificial intelligence
- deep learning
- human health
- stress induced
- rectal cancer
- oxide nanoparticles