A ratiometric ER calcium sensor for quantitative comparisons across cell types and subcellular regions.
Ryan J FarrellKirsten G BredvikMichael B HoppaS Thomas HenniganTimothy A BrownTimothy Aidan RyanPublished in: bioRxiv : the preprint server for biology (2024)
The endoplasmic reticulum (ER) is an important regulator of Ca 2 + in cells and dysregulation of ER calcium homeostasis can lead to numerous pathologies. Understanding how various pharmacological and genetic perturbations of ER Ca 2 + homeostasis impacts cellular physiology would likely be facilitated by more quantitative measurements of ER Ca 2 + levels that allow easier comparisons across conditions. Here, we developed a ratiometric version of our original ER-GCaMP probe that allows for more quantitative comparisons of the concentration of Ca 2 + in the ER across cell types and sub-cellular compartments. Using this approach we show that the resting concentration of ER Ca2+ in primary dissociated neurons is substantially lower than that in measured in embryonic fibroblasts.
Keyphrases
- endoplasmic reticulum
- estrogen receptor
- breast cancer cells
- high resolution
- living cells
- single cell
- quantum dots
- induced apoptosis
- spinal cord
- sensitive detection
- transcription factor
- genome wide
- gene expression
- hydrogen peroxide
- dna methylation
- oxidative stress
- copy number
- heart rate variability
- blood pressure
- bone marrow
- psychometric properties
- signaling pathway