Curcumin Ameliorated Oxidative Stress and Inflammation-Related Muscle Disorders in C2C12 Myoblast Cells.
Da-Yeon LeeYoon-Seok ChunJong-Kyu KimJeong-Ok LeeYoung Joon LeeSae Kwang KuSoon Mi ShimPublished in: Antioxidants (Basel, Switzerland) (2021)
The purpose of the current study was to investigate antioxidant and anti-inflammatory effects of spray dry powder containing 40% curcumin (CM-SD) in C2C12 myoblast cells. CM-SD increased DPPH radical scavenging activity in a dose-dependent manner, and up to 30 μg/mL of CM-SD did not express cytotoxicity in C2C12 cells. Exposure to hydrogen peroxide (H2O2) drastically decreased the viability of C2C12 cells, but pre-treatment of CM-SD significantly increased the cell viability (p < 0.01). CM-SD significantly transactivated the nuclear factor erythroid-2-related factor 2 (Nrf2)-dependent luciferase activity in a dose-dependent manner and enhanced the levels of heme oxygenase (HO)-1, glutamate cysteine ligase catalytic subunit (GCLC), and NAD(P)H-dependent quinone oxidoreductase (NQO)-1. CM-SD also significantly reduced reactive oxygen species (ROS) production and lipid peroxidation and restored glutathione (GSH) depletion in H2O2-treated C2C12 cells. Moreover, CM-SD significantly reduced lipopolysaccharides (LPS)-mediated interleukin (IL)-6 production in the conditioned medium. Results from the current study suggest that CM-SD could be a useful candidate against oxidative stress and inflammation-related muscle disorders.
Keyphrases
- induced apoptosis
- oxidative stress
- cell cycle arrest
- hydrogen peroxide
- endoplasmic reticulum stress
- anti inflammatory
- reactive oxygen species
- dna damage
- signaling pathway
- nuclear factor
- cell death
- skeletal muscle
- nitric oxide
- ischemia reperfusion injury
- inflammatory response
- combination therapy
- heat shock protein
- drug induced