Anticancer and Neuroprotective Activities of Ethyl Acetate Fractions from Morus macroura Miq. Plant Organs with Ultraperformance Liquid Chromatography-Electrospray Ionization-Tandem Mass Spectrometry Profiling.
Dalia Ibrahim HamdanSamia SalahWafaa Hassan Badr HassanMai MorsiHeba Muhammed Ali KhalilOmar Abdel-Hamed Ahmed-FaridRiham Adel El-ShiekhManal AbdElaziz NaelAhmed Mohamed ElissawyPublished in: ACS omega (2022)
Column chromatography afforded the isolation of seven secondary metabolites (1-(2,4,6-trihydroxy phenyl)-ethanone-4- O -β-d-glucopyranoside, naringenin-7- O -β-d-glucopyranoside, kaempferol-3- O -α-l-rhamnoside, kaempferol-3- O -β-d-glucopyranoside, quercetin-3- O -β-d-glucopyranoside, quercetin-3- O -β-d-galactopyranoside, rutin) from the ethyl acetate (ET) fractions of Morus macroura Miq. stems (S), leaves (L), and fruits (F). Their identification based on ultraviolet (UV), electron ionization (EI), electrospray ionization-mass spectrometry (ESI-MS), and 1D and 2D NMR data. In addition, profiling of ET fractions using ultraperformance liquid chromatography-electrospray ionization-tandem mass spectrometry (UPLC-ESI-MS/MS) resulted in the identification of 82 compounds belonging to different classes, mainly polyphenolic constituents. Chemical profiling as well as molecular docking directed us to biological evaluation. Interestingly, the ET-L fraction exhibited a robust cytotoxic activity against HepG-2, MCF-7, and HELA cell lines. Also, it displayed a neuromodulatory activity against cisplatin neurotoxicity in rats by ameliorating the neurobehavioral dysfunction visualized in the open field and Y-maze test and modulating the neurochemical parameters such as brain amino acid levels (glutamate, aspartate, serine, and histidine), oxidative stress markers (GSH, MDA, and 8-hydroxy-2'-deoxyguanosine), and purinergic cell energy (adenosine triphosphate (ATP) and adenosine monophosphate (AMP)). In conclusion, the isolated compounds (kaempferol-3- O -β-glucoside and quercetin-3- O -β-glucoside) from the ET-L fraction could serve as potent anticancer agents due to their strong antioxidant, in vitro cytotoxicity, and in vivo neuroprotective activity.
Keyphrases
- liquid chromatography
- tandem mass spectrometry
- mass spectrometry
- ms ms
- ultra high performance liquid chromatography
- simultaneous determination
- gas chromatography
- liquid chromatography tandem mass spectrometry
- high performance liquid chromatography
- high resolution mass spectrometry
- oxidative stress
- molecular docking
- solid phase extraction
- single cell
- high resolution
- protein kinase
- cerebral ischemia
- amino acid
- capillary electrophoresis
- breast cancer cells
- machine learning
- ionic liquid
- white matter
- electronic health record
- cell therapy
- minimally invasive
- mesenchymal stem cells
- brain injury
- multiple sclerosis
- cell cycle arrest
- data analysis
- blood brain barrier
- functional connectivity