Phenolic composition, antioxidant and antinociceptive activities of Syringa vulgaris L. bark and leaf extracts.
Erzsébet VargaCsenge BarabásAnita TóthImre BoldizsárBéla NoszálGergő TóthPublished in: Natural product research (2018)
Metabolite profile, antioxidant and antinociceptive activities of Syringa vulgaris bark and leaf methanolic extracts were investigated. By means of HPLC-DAD-ESI-TOF and HPLC-DAD-ESI-MS/MS, a total of 33 phenolics were identified, including 15 secoiridoids, 6 phenylpropanoids, 3 flavonoids, 3 lignans and 6 low molecular weight phenols. Validated quantitative analysis show that syringin (2.52%) and rutin (1.13%) are the main phenolic compounds in bark and leaf, respectively. Notable radical scavenging and antinociceptive activities of the bark and leaf extracts were confirmed by in vitro DPPH● and ABTS●+ assays and by in vivo hot-plate method in mice, respectively. Our results could lay the scientific basic of future clinical perspectives of lilac bark and leaf.
Keyphrases
- ms ms
- anti inflammatory
- liquid chromatography tandem mass spectrometry
- oxidative stress
- ultra high performance liquid chromatography
- high throughput
- mass spectrometry
- simultaneous determination
- skeletal muscle
- high resolution
- insulin resistance
- high fat diet induced
- tandem mass spectrometry
- single cell
- liquid chromatography
- solid phase extraction