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Target-activated cascade transcription amplification lights up RNA aptamers for label-free detection of metalloproteinase-2 activity.

Ning-Ning ZhaoWen-Jing LiuXiaorui TianBaogang ZhangShuangshuang Zhang
Published in: Chemical communications (Cambridge, England) (2023)
We demonstrate that target-activated cascade transcription amplification lights up RNA aptamers for label-free detection of metalloproteinase-2 (MMP-2) activity with zero background. This assay exhibits good specificity and high sensitivity with a limit of detection (LOD) of 0.6 fM. Moreover, it can analyze enzyme kinetic parameters, screen inhibitors, and accurately quantify MMP-2 in cancer cells and clinical serums.
Keyphrases
  • label free
  • nucleic acid
  • high throughput