Combined ion beam irradiation platform and 3D fluorescence microscope for cellular cancer research.
Andrew D HarkenNaresh T DeoliCitlali Perez CamposBrian PonnaiyaGuy GartyGrace S LeeMalte Johannes CasperShikhar DhingraWenze LiGary W JohnsonSally A AmundsonPeter W GrabhamElizabeth M C HillmanDavid J BrennerPublished in: Biomedical optics express (2024)
To improve particle radiotherapy, we need a better understanding of the biology of radiation effects, particularly in heavy ion radiation therapy, where global responses are observed despite energy deposition in only a subset of cells. Here, we integrated a high-speed swept confocally-aligned planar excitation (SCAPE) microscope into a focused ion beam irradiation platform to allow real-time 3D structural and functional imaging of living biological samples during and after irradiation. We demonstrate dynamic imaging of the acute effects of irradiation on 3D cultures of U87 human glioblastoma cells, revealing characteristic changes in cellular movement and intracellular calcium signaling following ionizing irradiation.
Keyphrases
- radiation induced
- radiation therapy
- induced apoptosis
- high speed
- high resolution
- cell cycle arrest
- endothelial cells
- early stage
- locally advanced
- optical coherence tomography
- low dose
- signaling pathway
- atomic force microscopy
- oxidative stress
- squamous cell carcinoma
- cell death
- respiratory failure
- hepatitis b virus
- energy transfer
- single cell