Transcriptome analysis of primary chicken cells infected with infectious bronchitis virus strain K047-12 isolated in Korea.
Rangyeon LeeJi Seung JungJi-In YeoHyuk Moo KwonJeongho ParkPublished in: Archives of virology (2021)
Infectious bronchitis virus (IBV), an avian coronavirus, is highly contagious. Chickens with IBV infection develop acute pathogenesis in multiple organs, including the respiratory and urogenital tracts. Frequent recombination in the spike (S) glycoprotein gene has made vaccine strategies ineffective. To understand IBV pathogenesis, we analyzed the genetic distance between Korean IBV isolates and other coronaviruses, including SARS-CoV-2. To obtain comprehensive information about early immune responses such as innate cytokine production and associated immune regulation during IBV infection, we infected primary chicken embryonic kidney cells and performed transcriptome analysis. We observed that the functional pathways of innate immunity are regulated and confirmed expression of genes that coordinate early immune responses. Understanding the immune profile of the host cell may assist in vaccine development.
Keyphrases
- immune response
- sars cov
- induced apoptosis
- genome wide
- cell cycle arrest
- toll like receptor
- single cell
- endoplasmic reticulum stress
- stem cells
- signaling pathway
- dna methylation
- dendritic cells
- cell therapy
- heat stress
- respiratory failure
- binding protein
- cell proliferation
- mesenchymal stem cells
- bone marrow
- long non coding rna
- mechanical ventilation
- aortic dissection