Identification and Quantification of Modified Nucleosides in Saccharomyces cerevisiae mRNAs.
Mehmet TarduJoshua D JonesRobert T KennedyQishan LinKristin D KoutmouPublished in: ACS chemical biology (2019)
Post-transcriptional modifications to messenger RNAs (mRNAs) have the potential to alter the biological function of this important class of biomolecules. The study of mRNA modifications is a rapidly emerging field, and the full complement of chemical modifications in mRNAs is not yet established. We sought to identify and quantify the modifications present in yeast mRNAs using an ultra-high performance liquid chromatography tandem mass spectrometry method to detect 40 nucleoside variations in parallel. We observe six modified nucleosides with high confidence in highly purified mRNA samples (N7-methylguanosine, N6-methyladenosine, 2'-O-methylguanosine, 2'-O-methylcytidine, N4-acetylcytidine, and 5-formylcytidine) and identify the yeast protein responsible for N4-acetylcytidine incorporation in mRNAs (Rra1). In addition, we find that mRNA modification levels change in response to heat shock, glucose starvation, and/or oxidative stress. This work expands the repertoire of potential chemical modifications in mRNAs and highlights the value of integrating mass spectrometry tools in the mRNA modification discovery and characterization pipeline.
Keyphrases
- saccharomyces cerevisiae
- heat shock
- liquid chromatography tandem mass spectrometry
- oxidative stress
- mass spectrometry
- binding protein
- genome wide analysis
- high resolution
- ms ms
- heat stress
- gene expression
- heat shock protein
- high throughput
- transcription factor
- climate change
- risk assessment
- induced apoptosis
- amino acid
- cell wall