A long-wavelength ultrasensitive colorimetric fluorescent probe for carbon monoxide detection in living cells.
Zuokai WangZiyang ZhaoCaiyun LiuZhuofan GengQingxia DuanPan JiaZilu LiHanchuang ZhuBao-Cun ZhuWenlong ShengPublished in: Photochemical & photobiological sciences : Official journal of the European Photochemistry Association and the European Society for Photobiology (2019)
Exploring techniques for monitoring the intracellular signaling molecule carbon monoxide (CO) in biosystems is important to help understand its various cellular functions. Therefore, a simple long-wavelength colorimetric fluorescent probe LW-CO was designed for selectively and sensitively detecting intracellular CO in living systems. Probe LW-CO is ultrasensitive and can track CO levels in the range of 0-1 μM, with a detection limit of about 3.2 nM. Additionally, the obvious color changes of probe LW-CO with CO (yellow to pink) provide a convenient way for on-site detection of CO with the naked eye. Probe LW-CO was applied to track the exogenous levels of CO in RAW264.7 cells. Probe LW-CO proved to be an efficient method for investigating various cellular functions of CO.
Keyphrases
- living cells
- fluorescent probe
- label free
- loop mediated isothermal amplification
- single molecule
- gold nanoparticles
- real time pcr
- quantum dots
- photodynamic therapy
- reactive oxygen species
- oxidative stress
- molecularly imprinted
- cell proliferation
- nitric oxide
- cell cycle arrest
- solid phase extraction
- endoplasmic reticulum stress
- tandem mass spectrometry