Ligation-based assay for variant typing without sequencing: Application to SARS-CoV-2 variants of concern.

Dalton J NelsonMeghan H ShiltsSuman B PakalaSuman R DasJonathan E SchmitzFrederick R Haselton

Published in: Influenza and other respiratory viruses (2022)

The assay demonstrates the potential for robust variant typing with performance comparable with next-generation sequencing without the need for the time delays and resources required for sequencing. The reduced resource dependency and generalizability could expand access to variant classification information for pandemic surveillance.

Keyphrases

sars cov
high throughput
single cell
copy number
coronavirus disease
respiratory syndrome coronavirus
machine learning
deep learning
public health
genetic diversity
gene expression
health information
risk assessment
climate change
cell free