Comparison of three methods to stabilize bronchoalveolar lavage cells for flowcytometric analysis.

Harrie H M EidhofJan W GratamaA H Leontine Mulder

Published in: Cytometry. Part B, Clinical cytometry (2020)

If analysis of the BAL cells is performed more than 24 hr after the lavage, stabilized BAL cells are superior to native cells. The in-house method can be used for EQA purposes with stability for at least 28 days. The TransFix and Streck methods might be useful for postponed diagnostic analysis of lavage cells but did not meet our 28 days criterion defined needed for EQA purposes.

Keyphrases

induced apoptosis
cell cycle arrest
cell death
oxidative stress
signaling pathway
cell proliferation
clinical evaluation