qPCR assay for the detection of pseudocowpox virus.

Gabriel Augusto de Oliveira LopesLuciana Rabello FerreiraGiliane de Souza TrindadeAntônio Augusto FonsecaJenner Karlisson Pimenta Dos Reis

Published in: Archives of virology (2020)

Pseudocowpox is a zoonosis caused by pseudocowpox virus (PCPV), which mainly affects cows but can be an occupational disease of humans. The aim of the study was to validate a quantitative polymerase chain reaction (qPCR) assay for the detection of PCPV. The assay was able to detect up to 1000 copies of PCPV per µL in field samples, with a sensitivity of 80% and a specificity of 100%. We did not observe any cross-reactivity between PCPV-positive samples and samples that were positive for other genetically similar viruses. The repeatability and reproducibility were adequate according to parameters preestablished in official test validation manuals.

Keyphrases

high throughput
loop mediated isothermal amplification
real time pcr
label free
high resolution
mass spectrometry
quantum dots