Ratiometric fluorescence determination of alkaline phosphatase activity based on carbon dots and Ce 3+ -crosslinked copper nanoclusters.
Rong-Mei KongPeihua LiXinyue GeYan ZhaoWeiheng KongMei-Hao XiangLian XiaFengli QuPublished in: Mikrochimica acta (2023)
A new ratiometric fluorescent probe for efficient determination of ALP was developed. The probe was constructed by combining Ce 3+ -crosslinked copper nanoclusters (Ce 3+ -CuNCs) which exhibit the aggregation-induced emission (AIE) feature with carbon dots (CDs). The introduction of phosphate (Pi) induced the generation of CePO 4 precipitation, resulting in significant decrease of fluorescence emission of CuNCs at 634 nm. At the same time, the fluorescence of CDs at 455 nm was obviously enhanced, thus generating ratiometric fluorescence response. Based on the fact that the hydrolysis of pyrophosphate (PPi) by ALP can produce Pi, the CD/Ce 3+ -CuNCs ratiometric probe was successfully used to determine ALP. A good linear relationship between the ratiometric value of F 455 /F 634 and ALP concentrations ranging from 0.2 to 80 U·L - 1 was obtained, with a low detection limit of 0.1 U·L - 1 . The ratiometric responses of the probe resulted in the visible fluorescence color change from orange red to blue with the increase of ALP concentration. The smartphone-based RGB recognition of the fluorescent sample images was used for ALP quantitative determination. A novel ratiometric fluorescent system based on Ce 3+ -CuNCs with AIE feature and CDs were constructed for efficient detection of ALP.
Keyphrases
- energy transfer
- fluorescent probe
- living cells
- quantum dots
- single molecule
- sensitive detection
- deep learning
- wastewater treatment
- solid phase extraction
- machine learning
- loop mediated isothermal amplification
- photodynamic therapy
- label free
- mass spectrometry
- molecularly imprinted
- liquid chromatography
- diabetic rats
- simultaneous determination
- optical coherence tomography
- neural network