Chromosomal damage in occupationally exposed health professionals assessed by two cytogenetic methods.

The study assessed occupationally induced chromosomal damage in hospital personnel at risk of exposure to antineoplastic drugs and/or low doses of ionizing radiation by two cytogenetic methods. Cultured peripheral blood lymphocytes of eighty-five hospital workers were examined twice over 2 to 3 years by classical chromosomal aberration analysis and fluorescence in situ hybridization. The comparison of the 1 st and the 2 nd sampling of hospital workers showed a significant increase in chromatid and chromosomal aberrations (all p  < .05) examined by classical chromosomal aberration analysis, and in unstable aberrations (all p  < .05) detected by fluorescence in situ hybridization. Both cytogenetic methods were able to detect an increase of unstable aberrations in the 2 nd sampling. The raised frequency of unstable cytogenetic parameters suggested higher recent exposure to genotoxic agents.