Transcriptomic signaling in zebrafish ( Danio rerio ) embryos exposed to environmental concentrations of glyphosate.
Luis Terrazas-SalgadoBeatriz Yáñez-RiveraRaúl Llera-HerreraAlejandra García-GascaIsabel Alvarado-CruzMiguel Betancourt-LozanoPublished in: Journal of environmental science and health. Part. B, Pesticides, food contaminants, and agricultural wastes (2022)
Glyphosate [N-(phosphonomethyl)glycine] is one of the most popular herbicides worldwide. Globally, the use of glyphosate is increasing, and its residues have been found in drinking water and food products. The data regarding the possible toxic effects of this herbicide are controversial. Therefore, the aim of this study was to evaluate the effects of glyphosate at environmental concentrations in zebrafish ( Danio rerio ) embryos. Embryos were exposed to 0, 1, 100, and 1,000 µg/L glyphosate for 96 h, and mortality, heart rate, and hatching rate were evaluated. After the experiment, RNA was extracted from the embryos for transcriptional analysis. No mortality was recorded, and exposure to 100 µg/L and 1,000 µg/L of glyphosate resulted in lower heart rates at 48 h. In addition, RNA-seq analysis revealed that glyphosate exposure induced subtle changes in gene transcription profiles. We found 30 differentially expressed genes; however, the highest glyphosate concentration (1,000 µg/L) induced the greatest number of differentially expressed genes involved in oocyte maturation, metabolic processes, histone deacetylation, and nervous system development.
Keyphrases
- rna seq
- heart rate
- drinking water
- single cell
- heart rate variability
- heart failure
- human health
- risk factors
- cardiovascular disease
- diabetic rats
- transcription factor
- type diabetes
- coronary artery disease
- atrial fibrillation
- copy number
- health risk
- genome wide identification
- artificial intelligence
- endothelial cells
- stress induced